Journal: MethodsX

Article Title: Simultaneous detection and quantification of spike mRNA and protein in SARS-CoV-2 infected airway epithelium .

doi: 10.1016/j.mex.2023.102050

Figure Lengend Snippet:

Article Snippet: HybEZ humidity control tray and HybEZ slide rack , Advanced Cell Diagnostics , N/A.

Techniques: Recombinant, Staining, Blocking Assay, Plasmid Preparation, Positive Control, Software, Membrane, Microscopy, Inverted Microscopy, RNAscope, Multiplex Assay, Control

Journal: Glia

Article Title: Neuroprotective effect of neuron‐specific deletion of the C16 ceramide synthetic enzymes in an animal model of multiple sclerosis

doi: 10.1002/glia.24631

Figure Lengend Snippet: Validation of the neuronal genetic ablation of both enzymes responsible for C16 ceramide synthesis. (a) Breeding scheme for the generation of mice with neuronal specific double deletion of CerS5 and CerS6 in CamK2a+ neurons ( CerS5/6 dKO). (b) qRT‐PCR analysis of CerS5 and CerS6 transcript levels in adult spinal cords from CerS5/6 dKO mice and their littermate controls. Data represent transcript values normalized to Gapdh and relative to values in controls ± SEM ( n = 6 controls and n = 6 CerS5/6 dKO. Unpaired two tailed t test, * p = .027. (c) Confocal images of in situ hybridization using Basescope and fluorescent probes (red) specific for either CerS5 or CerS6 in NeuN+ immunostained neurons (green) from Control and CerS5/6 dKO brain tissue. Scale bar represents 12.5 μm. (d,e) Bar graphs represent the proportion of NeuN+ cells with the indicated number of transcripts (referred as “dots”) for CerS5 (d) and CerS6 (e) transcripts. The number of red dots in NeuN+ cells was quantified for each probe and represented as relative percentage of cells. ( n = 3–4 mice). Two‐way ANOVA (probe × number of dots, p = .0005 for (d) and p = .004 for (e)), post‐hoc Sidak's multiple comparison test ** p < .01; *p < .05.

Article Snippet: BaseScope probes BaseScopeTM Probe—BA‐Mm‐ CerS5 ‐E4E5—targeting the Exon4‐Exon5 junction of CerS5 —and BaseScopeTM Probe—BA‐Mm‐ Cers6 ‐E4E5—targeting the Exon4‐Exon5 junction of CerS6 —were then hybridized for 2 h at 40°C in a humidity‐controlled oven (HybEZ II, ACDbio) before following the signal amplification steps provided by the manufacturer.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Two Tailed Test, In Situ Hybridization, Control, Comparison